Cell

Part:BBa_K2841503:Design

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-12)


Legionella Hemolysin Detector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 174
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constructed by primary sequence analysis and testing in NUPACK to obtain a structure that will complement Legionella pneumophila legiolysin RNA to become unfolded and activate the dCas9 it associates with


Source

Rodrigo, G., Landrain, T.E. and Jaramillo, A. (2012) De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A, 109, 15271-15276.

References