![](https://parts.igem.org/images/partbypart/icon_cell.png)
Cell
Part:BBa_K2841503:Design
Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside Group: iGEM18_Warwick (2018-10-12)
Legionella Hemolysin Detector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 145
Illegal NgoMIV site found at 174
Illegal NgoMIV site found at 408
Illegal NgoMIV site found at 437 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Constructed by primary sequence analysis and testing in NUPACK to obtain a structure that will complement Legionella pneumophila legiolysin RNA to become unfolded and activate the dCas9 it associates with
Source
Rodrigo, G., Landrain, T.E. and Jaramillo, A. (2012) De novo automated design of small RNA circuits for engineering synthetic riboregulation in living cells. Proc Natl Acad Sci U S A, 109, 15271-15276.